Figure 4

Uremic plasma inhibits VDR-RXRα-DR-3 complex formation and hemodialysis reduces this effect. Gel Shift experiments were performed using in vitro translated [³⁵S] hVDR and cold hRXRα. [³⁵S] hVDR was incubated with VD₃ vitamin for 30 min at 4°C and then without (Control – lane 1) or with increasing volumes (0.5, 1.0, 2.0 μL) of normal (lanes 2–4) or uremic plasma, collected before (pre-HD – lanes 5–7) and after hemodialysis (pos-HD – lanes 8–10) from patient 1, for 30 min. Cold DR-3 type VDRE (5'- AGCT TC AGGTCA AGG AGGTCA GAG - 3'), cold hRXRα, and nonspecific DNA poly (dIdC) were subsequently added and incubated for 20 min.