Figure 1From: Diurnal difference in CAR mRNA expressionDiurnal variations in CAR mRNA in the rat liver. Animals were sacrificed every 4 hours at Zeitgeber times (ZT) 4, 8, 12, 16, 20 and 24/0. mRNA levels of CAR, CYP2B1/2, BMAL1, REV-ERBĪ± and GAPDH were amplified by semi-quantitative RT-PCR. After oligo(dT)-primed cDNA was synthesized from rat liver total RNA, PCR was conducted with an initial enzyme activation step at 95Ā°C for 5 min followed by divergent cycles of denaturation at 95Ā°C for 15 sec, annealing at 60Ā°C for 30 sec and extension at 72Ā°C for 60 sec; CAR (27 cycles), CYP2B1/2, REV-ERBĪ± and BMAL1(30 cycles), and GAPDH (24 cycles). The reaction products were separated by agarose gel electrophoresis and stained with ethidium bromide.Back to article page