Figure 5
From: Binding of estrogen receptor with estrogen conjugated to bovine serum albumin (BSA)
E 2 competition with E 2 -BSA-FITC binding. MC7 cells (black bars) or HeLa-ERα (white bars) were incubated with vehicle, various concentrations of E2, or ICI 182,780 (10-8 M) for 30 minutes and then incubated an additional 30 minutes with E2-BSA-FITC or BSA-FITC alone (grey bar). Cells were fixed, and visualized by confocal microscopy. Digitized images were inverted to black on white and pixel density for each cell was determined by averaging the density across the cell membrane at four orthogonal points. Each bar represents >20 cells counted +/- SEM. (* p < 0.05).