Figure 1

Structure and properties of the bait RAR mutant and of one of the identified preys, PLZF. A) Schematic representation of the nuclear receptor RARα and structural localization of the two mutations K262A and K244A. These mutations weaken the interaction with the corepressor SMRT and abolish the interaction with the coactivator SRC-1, as visualized by GST pull-down assays (insert). B) Structure of the transcription factor PLZF identified by the two-hybrid screening of an ovary cDNA library with pLex12-RAR K244A-K262A used as a bait.