TSA Enhances ERβ-LBD activity. (A) Results of a HeLa cell transfection in which ERα and ERβ activity was compared at a simple ERE responsive reporter gene. The value obtained in the absence of ER and ligand was set at 1 (irrespective of the presence or absence of TSA, 100 nM). Other values were calculated relative to this value. A single representative experiment is shown with errors derived from three wells. (B) Results of transfection in which ERα and ERβ LBD activities were compared in the absence (left) or presence (right) of TSA (100 nM); note differences in scale. A single representative experiment is shown. Standard errors are derived from three wells.