Figure 2

Active Stat3 stimulates hormone-induced transcriptional activity of other SHRs. CV-1 cells were cotransfected with specific luciferase reporter plasmids (ARE-TATA-luciferase for GR and PR, and ERE-TATA-luciferase for ER), their corresponding SHR expressing plasmid (GR, top; PR, middle; and ER, bottom), and Stat3C plasmid or empty vector. After transfection, cells were treated for 36–40 h with 10 nM of the corresponding hormone (Dex for GR; R5020 for PR; and E₂ for ER) or 0.1% ethanol as vehicle in 5% charcoal stripped serum in phenol red-free DMEM. Luciferase activity was determined in cell extracts and corrected per μg of protein. Data are expressed as mean ± S.E. of three independent experiments.