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Figure 6 | Nuclear Receptor

Figure 6

From: Gene expression profiling of potential peroxisome proliferator-activated receptor (PPAR) target genes in human hepatoblastoma cell lines inducibly expressing different PPAR isoforms

Figure 6

Chromatin immunoprecipitation assays of the ADRP promoter. HepG2-tet-off-hPPAR cells were treated with PPAR ligands (100 μM fenofibric acid for PPARα, 100 nM GW501516 for PPARδ, or 10 μM troglitazone for PPARγ) for 8 h in the absence of Dox and processed for the ChIP assays. Soluble chromatin was immunoprecipitated with pre-immune rabbit IgG (lanes 2 and 6), anti-PPAR antibodies (lanes 3 and 7), or anti-RXRα antibody (lanes 4 and 8). Immunoprecipitates were subjected to PCR with a primer-pair specific to the ADRP promoter. As a negative control, a second set of primers were used to amplify another genomic region that was not expected to interact with the PPARs. PCR was performed with total chromatin input (lanes 1 and 5).

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Nuclear Receptor

ISSN: 1478-1336