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Figure 3 | Nuclear Receptor

Figure 3

From: Gene expression profiling of potential peroxisome proliferator-activated receptor (PPAR) target genes in human hepatoblastoma cell lines inducibly expressing different PPAR isoforms

Figure 3

Eight gene expressions were modulated by PPARs in tet-regulated HepG2 cells. A, B, C and D, HepG2-tet-off-hPPAR cells were treated with DMSO (vehicle) or PPAR ligands (100 μM fenofibric acid for PPARα (A), 100 nM GW501516 for PPARδ (B), 10 μM ciglitizone for PPARγ1 (C) or PPARγ2 (D)) for 0, 8, 24, 48 or 72 h in the absence of Dox. Messenger RNA levels of human ADRP, HMGCS2, HADHA, PEPCK, MCAD, ANGPTL4, L-FABP and CLAMP/PDZK1 were measured by real time RT-PCR. Values are expressed as mean ± S.E.M. (n = 3) target mRNA levels normalized to GAPDH mRNA.

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Nuclear Receptor

ISSN: 1478-1336