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Figure 5 | Nuclear Receptor

Figure 5

From: Thyroid hormone receptor binding to DNA and T3-dependent transcriptional activation are inhibited by uremic toxins

Figure 5

Uremic plasma does not decrease PPARγ-RXRα-DR-1 complex formation. Gel Shift experiments were performed using in vitro translated [35S] PPARγ and cold hRXRα. [35S] PPARγ was incubated without (Control – lane 1) or with increasing volumes (0.5, 1.0, 2.0 μL) of normal (lanes 2–4) or uremic (lanes 5–7) plasma for 30 minutes. Cold DR-1 type TRE (5'- AGCT TC AGGTCA G AGGTCA GAG - 3'), cold hRXRα, and nonspecific DNA poly (dIdC) were subsequently added and the reaction was incubated for 20 min.

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