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Figure 3 | Nuclear Receptor

Figure 3

From: Thyroid hormone receptor binding to DNA and T3-dependent transcriptional activation are inhibited by uremic toxins

Figure 3

Hemodialysis reduces the inhibitory effect of uremic plasma on hTRβ1-hRXRα binding to DR-4. [35S] hTRβ1 was pre-incubated without (Control – lane 1) or with increasing volumes (0.5, 1.0, 2.0 μL) of normal (lane 2–4) or uremic plasma (patient 1) collected before (pre-HD – lanes 5–7) or after hemodialysis (pos-HD – lanes 8–10) for 30 min at 4°C. Cold DR-4 type TRE, cold hRXRα, and nonspecific DNA poly (dIdC) were subsequently added and incubated for 20 min.

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