Figure 1
Uremic plasma reduces hTRβ1-hRXRα complex formation on DR-4. Gel Shift experiments were performed using in vitro translated [35S] hTRβ1, cold hRXRα and DR-4. [35S] hTRβ1 was treated with T3 10-7M for 30 min at 4°C and then incubated without (Control – lane 1) or with increasing volumes (0.5, 1.0, 2.0 μL) of normal (lanes 2–4) or uremic (lanes 5–7) plasma for 30 min at 4°C. Cold DR-4 type TRE (5'- AGCT TC AGGTCA CAGG AGGTCA GAG - 3'), cold hRXRα, and nonspecific DNA poly (dIdC) were subsequently added and incubated for 20 min.