Figure 5

Nuclear CXR protein levels decrease after PB-treatment. LMH cells were treated with vehicle or 400 μM PB for 16 hours before cells were lysed and CXR protein levels in the total lysate (lanes 1 and 2) and in nuclear extracts (lanes 3 and 4) determined by Western blot. As controls, CV-1 cells were transfected with control vector or CXR expression vector (lanes 5 and 6). MW, molecular weight in kDa.