Figure 3
Diurnal difference of CYP2B induction. Animals were sacrificed at ZT13 and ZT25/1 5-hour after the injection of PB (gray columns) or veihcle (black columns) during ZT8-13 [Day] and ZT20-1 [Night], respectively. Oligo(dT)-primed cDNA was synthesized from rat liver total RNA from each animal, and CYP2B mRNA levels were measured by STBR Green real-time RT-PCR. The results were normalized against those of GAPDH. The columns and bars represent the means Ā± SD with significant differences compared to the individual controls at *, #: p < 0.05