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Figure 6 | Nuclear Receptor

Figure 6

From: Binding of estrogen receptor with estrogen conjugated to bovine serum albumin (BSA)

Figure 6

Stability of E 2 -BSA-FITC. E2-BSA-FITC (10-8 M in estrogen) was placed in empty wells or wells containing MC7 cells and incubated for 30 minutes at 4°C. E2-BSA-FITC was detected by reverse phase HPLC using a methanol-water gradient from 80% methanol to 50% methanol over 30 minutes at 1.0 ml/min. The assay was run in triplicate. Representative spectra are shown for E2-BSA-FITC alone (A) and E2-BSA-FITC incubated with MC7 cells (B).

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