Figure 2

Whole cell binding of estradiol to MC7 cells and HeLa cells stably transfected with ERα. 2 × 10⁶ cells were incubated with ³H-17β-estradiol (10^-8 M) in the absence (solid) and presence (white) of 100 fold excess of unlabeled estradiol for 15 minutes at room temperature, washed, and placed on ice for 30 minutes. Cells were then pelleted, lysed and counted. Results are expressed as the mean +/- the SEM of 3 experiments (* p < 0.01)