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Figure 1 | Nuclear Receptor

Figure 1

From: Binding of estrogen receptor with estrogen conjugated to bovine serum albumin (BSA)

Figure 1

Pre-incubation of purified hERα with E 2 -BSA competes for estradiol binding. Purified ERα was incubated with E2 (solid line) or E2-BSA (dotted line) for four hours before (a) or concurrently with (b) the addition of labeled E2. Incubation was continued for another 2 h at room temperature, and at the end of this period, specific binding was determined by adsorption, removal, and counting of free labeled E2.

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