Figure 3

LPS reduces binding activities of RXRα-containing heterodimer pairs to canonical DNA elements. Electrophoretic mobility shift assay analysis of hepatic nuclear extracts prepared from C57BL/6 mice injected with control saline or 2 μg/g bw LPS for 16 h. Radiolabeled double-stranded DR1, DR2, DR3, DR4 and DR5 elements or a consensus AP1 element were employed (see Materials and Methods). The samples were electrophoresed through a 6% non-denaturing polyacrylamide gel, dried and analyzed by autoradiography.