Figure 4
From: Estrogen receptor-dependent activation of AP-1 via non-genomic signalling
Intact MAP-kinase activity is important for non-genomic actions of ERs at AP-1 sites. (A) HC11 cells were transiently co-transfected with 500 ng coll-73-luc reporter gene and 200 ng of NLSA, ERβ 148-530 Flag or C201A/C204A. Cells were pretreated with 10 μM UO126 or 10 μM LY294002 for one hour before the addition of 10-8 M 17β-estradiol (E2), and the reporter activity was analysed 6 hours after treatment. (B) SYF cells were transiently co-transfected with 500 ng coll-73-luc reporter gene and 200 ng of NLSA, ERβ 148-530 Flag or C201A/C204A. Cells were treated with either no hormone (NH), 10-8 M 17β-estradiol (E2) or 10-7 M tamoxifen (OHT), and the reporter activity was analysed 24 hours after treatment. Luciferase activity was normalised using β-gal as an internal control. Data are representative of at least three independent experiments performed in duplicate. Mean and ± SD are shown.