Table 1 Oligonucleotides used for RT-PCR analysis
Primer | Direction | Sequence (5'-3') | Exon | Detected variants/Fragment size |
|---|---|---|---|---|
F1 | forward | TGG AGT TGC TCT TTC ACT TCC | 7 | SV 1, 3, 5, 9/121 bp |
R1 | reverse | TCT CTC TGG GTA ACT CCA GGT C | 8 | SV 2, 6, 8, 10/136 bp |
F2 | forward | GAC CTG GAG TTA CCC AGA GAG A | 8 | SV 1, 2, 3, 4, 6, 7/211 bp |
R2 | reverse | ATC TCC TGG AGC AGC GGC ATC AT | 9 | SV 5, 8, 9, 10, 11, 12/135 bp |
F3 | forward | CTA CCA CTT TAA TGC GCT GAC T | 2 | Â |
|  |  |  |  | SV 1–12 (all variants)/121 bp |
R3 | reverse | CCT CTG AGT CTT GCT GAC TTC | 3 | Â |