Figure 3From: The peroxisome proliferator activated receptor δ is required for the differentiation of THP-1 monocytic cells by phorbol esterCompound F modulates phorbol ester activation of gene targets involved in differentiation. Cells were plated in RPMI 1640 supplemented with 0.5% FCS. A PMA dose-response was carried out, in the presence of either vehicle alone (squares, broken lines) or compound F (triangles, solid lines). Cells were lyzed after 24 h (CD68; A, IL8; B, AFABP; C and adipophilin; D) or 48 h (IL1β ; E, Osteopontin; F and MMP-9; G). RNA was prepared from cell lysates and cDNA was prepared for QPCR analysis.Back to article page