Figure 6

NPDC-1 Alters RARβ••••α and RXRα DNA binding properties. Oligonucleotides corresponding to the consensus RARβ•RARE (DR-5: A) or the consensus RXRα RARE (DR-1: B & C) were end-labeled with ^32-P by T4 polynucleotide kinase according to the manufacture's instructions. The resultant DNA binding probes were incubated with PC12 lysate (A), recombinant NPDC-1 and recombinant RXRα (B & C) as indicated. DNA:protein complexes were resolved on non-denaturing PAGE gels which were subsequently exposed to Kodak Xar-5 film. In C, the presence of NPDC-1 within the RXR gel-shift complex was assayed by supershift. Antibodies specific for either NPDC-1 or RXR were used to identify proteins present in the complex.