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Figure 1 | Nuclear Receptor

Figure 1

From: Expression and localization of P1 promoter-driven hepatocyte nuclear factor-4α (HNF4α) isoforms in human and rats

Figure 1

Schematic illustration of the human HNF4α gene, gp64 fusion proteins and Western blot analysis of HNF4α protein using K9218 made to the human HNF4α1/α2/α3 A/B domain. (A) Human HNF4α gene structure. Shown are the 13 exons of the human HNF4α gene. In the human genome, an alternative promoter referred to as P2 promoter is located 45.6 kb from the previously characterized P1 promoter. Exons are shown as boxes. (B) Schematic representation of HNF4α isoforms and gp64 fusion proteins. The A/B domains of HNF4α1 /α2/α3 are encoded by exon 1A and by exon 2 for the last 19 amino acids. The amino terminal amino acids of HNF4α1/2/3 were inserted between the signal peptide and gp64 mature domain. (C) The specificity of K9218 was tested by Western blot analysis using HNF4α2- and HNF4α3-transfected CHO and cultured cells. Whole cell extracts from CHO cells transfected with human HNF4α2 and HNF4α3 (4 μg) or cultured cells (20 μg) were utilized for detection of the HNF4α.

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