Figure 4

ERβ binds the N-CoR C-terminus. Binding of ERβ to short bacterially produced GST-N-CoR fragments was assessed as in Fig. 1B. The approximate positions of N-CoR IDs are indicated with black bars, the putative ERβ interacting motif with a grey bar. Note that all of the binding experiments with shorter N-CoR fragments were performed in parallel, and are compared to the same input ERβ protein (10% of total) displayed alongside the 1944–2033 fragment. A binding experiment that was performed in parallel utilizing the entire GST-N-CoR C-terminus (1944–2453) is shown for comparison at top. The likely reasons that ERβ binding to the shorter N-CoR fragment (2322–2453) is stronger than that obtained with the intact N-CoR C-terminus are discussed in the text.