Figure 3

Active Stat3 stimulates AR transcriptional activity similarly as and synergistically with other coactivators. CV-1 cells were cotransfected with ARE-TATA-luciferase, AR, and Stat3C or different coactivators (SRC-1, CBP/p300, TIF2 or P/CAF), individually or in combination. All wells contained the same total amount of DNA. After transfection, cells were treated for 36–40 h with 10 nM R1881 or 0.1% ethanol as vehicle in 5% charcoal stripped serum in phenol red-free DMEM. Luciferase activity was determined in cell extracts and corrected per μg of protein. Data are expressed as mean ± S.E. of three independent experiments.