Figure 4From: Complex effects of rexinoids on ligand dependent activation or inhibition of the xenobiotic receptor, CARRexinoid effects on expression of CYP2B6 in mCAR expressing HepG2 cells. Total RNA was prepared from a previously described HepG2 derivative stably expressing mCAR [16] and cultured in the presence of the indicated ligands (An, androstanol; LG, LG1069; TC, TCPOBOP) for 9 hours. Expression of CYP2B6 and the EF1α control was analyzed by northern blotting using appropriate cDNA probes and quantitated by phosphorimager analysis. Very similar results were obtained in 3 independent experiments; a representative experiment is shownBack to article page