Figure 4

Stat3 does not affect specificity of AR for non-androgen steroid hormones or non-androgen responsive elements. (Top) CV-1 cells were cotransfected with ARE-TATA-luciferase, AR, and Stat3C or empty vector, and treated for 36–40 h with 10 nM each different steroid hormones (R1881, Dex, R5020, or E₂) or 0.1% ethanol as vehicle in 5% charcoal stripped serum in phenol red-free DMEM. (Bottom) CV-1 cells were cotransfected with ARE-TATA-luciferase (left) or ERE-TATA-luciferase (right), AR, and Stat3C or empty vector, and treated for 36–40 h with 10 nM R1881 or 0.1% ethanol as vehicle in 5% charcoal stripped serum in phenol red-free DMEM. Luciferase activity was determined in cell extracts and corrected per μg of protein. Data are expressed as mean ± S.E. of three independent experiments.