Figure 1

Active Stat3 stimulates androgen-induced AR transcriptional activity. CV-1 cells were cotransfected with AR responsive reporter plasmids, ARE-TATA-luciferase (A, B) or PB(-286/+28)-luciferase (C), AR, and Stat3C, S tat3, or empty vector. After transfection, cells were treated for 36–40 h with 10 nM R1881 or 0.1% ethanol as vehicle in 5% charcoal stripped serum in phenol red-free DMEM. Luciferase activity was determined in cell extracts and corrected per μg of protein. Data are expressed as mean ± S.E. of three independent experiments.